
TY  - JOUR
A1  - Helliwell, Chris A. 
A1  - Olive, Mark R. 
A1  - Forster, Leigh Gebbie Richard 
A1  - Peacock, W. James 
A1  - Dennis, Elizabeth S. 
T1  - Isolation of an <emph type="2">ent</emph>-kaurene oxidase cDNA from <emph type="2">Cucurbita maxima</emph>
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2000
VL  - 27
IS  - 12
SP  - 1141
EP  - 1149
KW  - 
UR  - https://doi.org/10.1071/PP00079 
N2  - Four distinct cDNAs isolated from developing Cucurbita maxima Duchesne seeds included CYP701A1, a member of the same subfamily of cytochrome P450 enzyme as the Arabidopsis ent-kaurene oxidase GA3 which catalyses an early step in gibberellin (GA) biosynthesis. We have shown by complementation of the Arabidopsis ga3-2 mutant that the CYP701A1 cDNA encodes an ent-kaurene oxidase activity. Another cDNA, CYP88A2, encodes a protein which is in the same subfamily as the maize Dwarf3 protein and is likely to encode another GA biosynthetic enzyme catalysing a reaction between ent-kaurenoic acid and GA12. 
ER  -

