
TY  - JOUR
A1  - Wang, Ming-Bo 
A1  - Abbott, David C. 
A1  - Upadhyaya, Narayana M. 
A1  - Jacobsen, John V. 
A1  - Waterhouse, Peter M. 
T1  - <emph type="2">Agrobacterium tumefaciens</emph>-mediated transformation of an elite Australian barley cultivar with virus resistance and reporter genes
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2001
VL  - 28
IS  - 2
SP  - 149
EP  - 156
KW  - Schooner, BYDV, GFP, hpt, bar.
UR  - https://doi.org/10.1071/PP00103 
N2  - Efficient transformation of barley cv. Schooner was achieved using Agrobacterium delivery, hygromycin or bialaphos selection and embryogenic callus. Using this system, transgenic plants were generated that contained either the green fluorescent protein gene, or transgenes derived from barley yellow dwarf (BYDV) and cereal yellow dwarf (CYDV) viruses. Many of these plants contained 1&#8211;3 transgene copies that were inherited in a simple Mendelian manner. Some plants containing BYDV and/or CYDV derived transgenes showed reduced virus symptoms and rates of viral replication when challenged with the appropriate virus. The ability to transform Schooner is a significant advance for the Australian barley industry, as this elite malting variety is, and has for the last 15 years been, the most widely grown barley variety in eastern Australia. 
ER  -

