
TY  - JOUR
A1  - Wood, Craig C. 
A1  - Islam, Nazrul 
A1  - Ritchie, Raymond J. 
A1  - Kennedy, Ivan R. 
T1  - A simplified model for assessing critical parameters during associative <emph type="7">15</emph>N<emph type="8">2</emph> fixation between <emph type="2">Azospirillum</emph> and wheat
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2001
VL  - 28
IS  - 9
SP  - 969
EP  - 974
KW  - ammonia excretion, BNF, malate, 15N.
UR  - https://doi.org/10.1071/PP01036 
N2  - This paper originates from an address at the 8th International Symposium on Nitrogen Fixation with Non-Legumes, Sydney, NSW, December 2000 Detailed studies in field experiments have shown repeatedly that the transfer of 15N2 fixed by diazotrophic bacteria to wheat tissue is minimal. Here, a simple and convenient laboratory co-culture model was designed to assess important features of the association between Azospirillum brasilense and wheat, such as the rate of nitrogen fixation (acetylene reduction), ammonia excretion from the bacterium and the transfer of newly fixed 15N2 from the associative diazotroph to the shoot tissue of wheat plants. After 70 h, in this model, insignificant amounts of newly fixed N2 were transferred from an ammonia-excreting strain of A. brasilense to the shoot tissue of wheat. However, when malate was added to the co-culture the 15N enrichment of the shoot tissue increased 48-fold, indicating that 20% of shoot N had been derived from N2 fixation. Thus, the inability of the host plant to release carbon in the rhizosphere is a significant constraint in the development of associative N2-fixing systems. These specific results suggest that wheat plants with an increased release of photosynthate to the rhizosphere should be a priority for the future development of broad-acre agricultural systems that are more self-sufficient for nitrogen nutrition. The simplicity of the model for assessing the critical parameters of associative 15N2 fixation may allow large-scale surveys of plant&#8211;bacterial interactions to be conducted and a selection of improved associations for further study. 
ER  -

