
%0 Journal Article
%A Furbank, Robert T. 
%A Scofield, Graham N. 
%A Hirose, Tatsuro 
%A Wang, Xin-Ding 
%A Patrick, John W. 
%A Offler, Christina E. 
%T Cellular localisation and function of a sucrose transporter <emph type="2">OsSUT1</emph> in developing rice grains
%J Functional Plant Biology
%D 2001/12/13
%V 28
%N 12
%P 1187-1196
%K developing grain, H+-ATPase, rice, sucrose/proton symport, sucrose transport.
%R https://doi.org/10.1071/PP01111
%U https://www.publish.csiro.au/paper/PP01111 
%X We previously reported the cloning and tissue-specific expression of a gene encoding a sucrose/proton symporter in rice (Oryza sativa L.), OsSUT1 (Hirose et al. 1997, Plant Cell Physiology38, 1389&#8211;1396). This gene is expressed at high levels in the filling grain, leaf sheath and stem. Expression in these tissues occurred only after heading i.e. during the development of the reproductive structure as a major sink. In this paper, we report localisation of the transcript and protein to specific cells in the filling rice grain by in situ hybridisation with a probe from theOsSUT1 cDNA, and immunolocalisation of OsSUT1 and proton-pumping ATPase (H+-ATPase). An OsSUT1 cDNA probe recognises a transcript of approximately 2.4 kb, and the SUT1 antibody recognises a protein of approximately 55 kDa in total membrane protein extracts from the filling grain, leaf sheath and stem. In the developing grain, OsSUT1 is expressed at low levels before heading, with expression reaching a peak approximately ten days after emergence of the panicle from the sheath. Transcript is then present throughout seed development, with expression falling substantially after about 25 days post-heading. Both transcript and protein are localised to the aleurone cells of the developing grain, and are also detected in the maternal tissue, particularly the nucellus, vascular parenchyma tissue and the nucellar projection. Tissue slices from filling rice grain showed high rates of sucrose uptake that were inhibited by pCMBS. The role of OsSUT1 in sucrose transport to the filling grain endosperm is discussed. 
