
%0 Journal Article
%A González, Esteban Roberto 
%A Andrade, Alexander de 
%A Bertolo, Ana Letícia 
%A Lacerda, Gisele Coelho 
%A Carneiro, Raphael Tozelli 
%A Defávari, Valéria A. Prado 
%A Labate, M&ocirc;nica T. Veneziano 
%A Labate, Carlos Alberto 
%T Production of transgenic <emph type="2">Eucalyptus grandis</emph> x <emph type="2">E. urophylla</emph> using the sonication-assisted <emph type="2">Agrobacterium</emph> transformation (SAAT) system
%J Functional Plant Biology
%D 2002/01/16
%V 29
%N 1
%P 97-102
%K Eucalyptusspp., GUS, Lhcb1*2, regeneration, transgenic plants, ultrasound.
%R https://doi.org/10.1071/PP01142
%U https://www.publish.csiro.au/paper/PP01142 
%X A method for genetic transformation of germinating seeds and seedlings of Eucalyptus grandis &#215; E. urophyllais described using the sonication-assisted Agrobacterium-mediated transformation (SAAT) system. Seeds germinated for 2 d, and 15-d-old seedlings, sonicated for 30 s, had the highest percentage of &#946;-glucuronidase (GUS) transient expression (21.7 and 37.4%, respectively). Pre-sonication greatly enhanced the efficiency of transformation. The differential transformation of tissues was also investigated, with seeds imbibed for 2 d having over 90% of the blue sectors localised in cotyledons and in the intersection of the hypocotyls and roots, whereas in 5-d-old seedlings, 70% of GUS activity was detected in cotyledons. However, 15&#8211;17-d-old seedlings had around 60% of transformed sectors localised in the first pair of leaves. The efficiency of the method was also assessed using a chimeric construct containing the Lhcb1*2 gene of the 28 kDa chlorophyll a/b binding pea protein from the LHCII antenna. Four stable transformants were confirmed by genomic blotting. 
