
TY  - JOUR
A1  - Bowes, George 
A1  - Rao, Srinath K. 
A1  - Estavillo, Gonzalo M. 
A1  - Reiskind, Julia B. 
T1  - C<emph type="8">4</emph> mechanisms in aquatic angiosperms: comparisons with terrestrial C<emph type="8">4</emph> systems
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2002
VL  - 29
IS  - 3
SP  - 379
EP  - 392
KW  - C3&#8211;C4 intermediate, C4 photosynthesis, CO2 concentrating mechanism, Egeria, Eleocharis, Hydrilla, Kranz anatomy, NADP-ME, Neostapfia, Orcuttia, phosphoenolpyruvate carboxylase, Rubisco, Sagittaria, Thalassiosira, Tuctoria, Udotea.
UR  - https://doi.org/10.1071/PP01219 
N2  - Aquatic C4 photosynthesis probably arose in response to dissolved CO2 limitations, possibly before its advent in terrestrial plants. Of over 7600 C4 species, only about a dozen aquatic species are identified. Amphibious Eleocharis species (sedges) have C3&#8211;C4 photosynthesis and Kranz anatomy in aerial, but not submersed, leaves. Aquatic grasses have aerial and submersed leaves with C4 or C3&#8211;C4 photosynthesis and Kranz anatomy, but some lack Kranz anatomy in the submersed leaves. Two freshwater submersed monocots, Hydrilla verticillata and possibly Egeria densa, are C4 NADP-malic enzyme (NADP-ME) species. A marine macroalga, Udotea flabellum (Chlorophyta), and possibly a diatom, are C4, so it is not confined to angiosperms. Submersed C4 species differ from terrestrial in that &#946;-carboxylation is cytosolic with chloroplastic decarboxylation and Rubisco carboxylation, so the C4 and Calvin cycles operate in the same cell without Kranz anatomy. Unlike terrestrial plants, Hydrilla is a facultative C4 that shifts from C3 to C4 in low &#91;CO2&#93;. It is well documented, with C4 gas exchange and pulse-chase characteristics, enzyme kinetics and localization, high internal &#91;CO2&#93;, relative growth rate, and quantum yield studies. It has multiple phosphoenolpyruvate carboxylase isoforms with C3-like sequences. Hvpepc4 appears to be the photosynthetic form induced in C4 leaves, but it differs from terrestrial C4 isoforms in lacking a C4 signature Serine. The molecular mass of NADP-ME (72 kDa) also resembles a C3 isoform. Hydrilla belongs to the ancient Hydrocharitaceae family, and gives insight to early C4 development. Hydrilla is an excellent &#8216;minimalist&#8217; system to study C4 photosynthesis regulation without anatomical complexities. 
ER  -

