
%0 Journal Article
%A Ma, Qing-Hu 
%A Zhang, Ren 
%A Hocart, Charles H. 
%A Letham, David S. 
%A Higgins, Thomas J. V. 
%T Seed-specific expression of the isopentenyl transferase gene (<emph type="2">ipt</emph>) in transgenic tobacco
%J Functional Plant Biology
%D 1998/03/30
%V 25
%N 1
%P 53-59
%K 
%R https://doi.org/10.1071/PP96072
%U https://www.publish.csiro.au/paper/PP96072 
%X The Agrobacterium tumefaciens gene encoding isopentenyl transferase (ipt), a cytokinin biosynthetic gene, was fused to a promoter from a seed-specific gene, vicilin, and introduced into tobacco cells. Intact fertile plants were generated. The expression of the vicilin-ipt gene was shown to be confined to seed and resulted in enhanced levels of cytokinins in the developing seeds and increased seed protein content. Using a simplified quantification method, a significant increase in the levels of endogenous cytokinins was recorded at 16&#x2013;21 days after flowering. The growth of the transgenic plants and the development of the seeds appeared to be normal. 
