
TY  - JOUR
A1  - Richardson, Kim 
A1  - Fowler, Sarah 
A1  - Pullen, Carly 
A1  - Skelton, Caryl 
A1  - Morris, Bret 
A1  - Putterill, Jo 
T1  - T-DNA tagging of a flowering-time gene and improved gene transfer by <emph type="2">in planta</emph> transformation of <emph type="2">Arabidopsis</emph>
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1998
VL  - 25
IS  - 1
SP  - 125
EP  - 130
KW  - Arabidopsis, T-DNA tagging, in planta transformation, flowering-time gene, GIGANTEA.
UR  - https://doi.org/10.1071/PP97109 
N2  - Gene tagging with insertional mutagens greatly facilitates the isolation of novel genes. A new collection of Arabidopsis T-DNA tag insertion lines (n=2165) was generated by in planta transformation. Whole plants were vacuum-infiltrated in a suspension of Agrobacterium carrying the pGKB5 tagging vector. The efficiency of transformation increased with addition of the surfactant Silwet L-77 (0.005% v/v) to the Agrobacterium suspension. Visual screens of the T-DNA lines identified two mutants with floral defects. Allelism tests suggested that a mutation in the GIGANTEA gene was responsible for the late-flowering phenotype of one of the mutants. Linkage analysis indicated that the GIGANTEA gene was tagged in this mutant. 
ER  -

