
%0 Journal Article
%A Grof, Christopher P.L. 
%A Knight, Deon P. 
%A McNeil, Scott D. 
%A Lunn, John E. 
%A Campbell, James A. 
%T A modified assay method shows leaf sucrose-phosphate synthase activity is correlated with leaf sucrose content across a range of sugarcane varieties
%J Functional Plant Biology
%D 1998/06/26
%V 25
%N 4
%P 499-502
%K Saccharum, leaf, sucrose-phosphate synthase, SPS, sucrose, sugarcane.
%R https://doi.org/10.1071/PP97169
%U https://www.publish.csiro.au/paper/PP97169 
%X Eight different commercial and breeding varieties of sugarcane (Saccharum spp.) grown in controlled conditions were assayed for leaf sucrose-phosphate synthase (SPS) (EC 2.1.4.14) activity and leaf sucrose content. Leaf SPS activity measured at 25&#x02DA;C ranged between 0.06 and 0.14 nmol sucrose formed mg protein -1 min-1. The cross-varietal average for leaf SPS activity was 0.10 nmol &#181;g protein-1 min-1 (equivalent to 63.4 &#181;mol h-1 g FW-1 or 17.6 nkat g FW-1) which is consistent with previously published leaf SPS activities for sugarcane; however, previous studies have assayed leaf SPS activity at either 30 or 37&#x02DA;C. The range of leaf sucrose content across varieties (5.5&#8211;18.0 mg sucrose g FW-1, average 11.3 mg g FW-1) was consistent with all but one of four previously published reports. Leaf SPS activity and leaf sucrose content were significantly correlated across the eight varieties examined (r2 =0.877, d.f. =7,P&#60;0.001). Whilst previous reports have indicated a co-relationship between leaf SPS activity and leaf sucrose content in single sugarcane varieties both diurnally and with different nutrient regimes, this study shows, for the first time, that this co-relationship also holds true across a range of sugarcane varieties. 
