
TY  - JOUR
A1  - Leblova, S 
T1  - Isolation and Partial Characterization of Alcohol Dehydrogenase From Broad Bean (<I>Vicia faba</I>)
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1974
VL  - 1
IS  - 4
SP  - 579
EP  - 582
KW  - 
UR  - https://doi.org/10.1071/PP9740579 
N2  - Alcohol dehydrogenase isolated from broad bean was found to have a Km value of 1.0 &#215; 1.0 -2 M, a pH optimum of 8.7 and a molecular weight of  60 000 ± 5000. The enzyme lost 55 % of its activity after being heated at 55ºC, and was totally inactivated at 70&#176;C. Thermal stability of the enzyme was not enhanced by NAD+ or ethanol.    The substrate specificity of the enzyme is reported. Cysteine and mercaptoethanol activated the enzyme, whilep-chloromercuribenzoate, Cu2+, Hg2+, B4O72- -, Zn2+ and EDTA inhibited it. The influence of ethanol, acetaldehyde and growth substances on alcohol dehydrogenase activity in germinating broad bean seeds and plant tissues was also studied. 
ER  -

