
%0 Journal Article
%A Steveninck, RFMV 
%A Ballment, B 
%A Peters, PD 
%A Hall, TA 
%T Ultrastructural Localization of Ions. II. X-Ray Analytical Verification of Silver Precipitation Products and Distribution of Chloride in Mesophyll Cells of Barley Seedlings
%J Functional Plant Biology
%D 1976/06/01
%V 3
%N 3
%P 359-365
%K 
%R https://doi.org/10.1071/PP9760359
%U https://www.publish.csiro.au/paper/PP9760359 
%X The ultrastructural localization of Cl- by means of a silver precipitation technique has been verified by means of X-ray analytical electron microscopy (EMMA-4 electron microscope plus KEVEX energy dispersive analyser). It was shown that the localization method was specific for Cl- in the case of electron-dense deposits in chloroplasts, plasmodesmata and plasmalemma of barley mesophyll cells.    It was pointed out that the use of nitric acid rinses in order to remove non-specific deposits of silver phosphates and carbonates has pitfalls and that the precipitation technique should always be used in conjunction with X-ray microprobe analysis. 
