
%0 Journal Article
%A Osmond, CB 
%A Bender, MM 
%A Burris, RH 
%T Pathways of CO<sub>2</sub> Fixation in the Cam Plant <I>Kalanchoe daigremontiana</I>. III. Correlation With &#948;<sup>13</sup>C Value During Growth and Water Stress
%J Functional Plant Biology
%D 1976/12/01
%V 3
%N 6
%P 787-799
%K 
%R https://doi.org/10.1071/PP9760787
%U https://www.publish.csiro.au/paper/PP9760787 
%X Seedlings of K. daigremontiana were grown in the same controlled-environment room under nine different day temperature and light regimes. In each treatment, irrigated plants maintained dawn water potentials of approximately -5 bar whereas droughted plants ranged to -22 bar. Irrigated plants showed a characteristic stomatal opening on illumination, closure during deacidification and partial stomatal opening following deacidification. Stomata of droughted plants remained closed throughout the light period. Irrigated and droughted plants showed comparable dark acid synthesis. Low light levels and low day temperature resulted in lower dark acid synthesis and more protracted deacidification in the light. The &#948;13C value (total carbon) of old as well as newly formed leaves of irrigated plants tended to become less negative throughout the experiment except in low-light, low-day-temperature treatments. This trend was accentuated in the &#948;13C value (total carbon) of whole shoots of droughted plants. These data are consistent with an increase, with age, in the proportion of CO2 assimilated in the dark during growth of irrigated plants, and with the greater dependence on dark CO2 assimilation during the slower growth of droughted plants. Differences in the &#948;13C value of soluble and insoluble carbon from irrigated and droughted plants assayed at dawn appear to be consistent with the established starch to acid relationships in crassulacean plants. 
