
%0 Journal Article
%A Lott, JNA 
%A Buttrose, MS 
%T Globoids in Protein Bodies of Legume Seed Cotyledons
%J Functional Plant Biology
%D 1978/02/01
%V 5
%N 1
%P 89-111
%K 
%R https://doi.org/10.1071/PP9780089
%U https://www.publish.csiro.au/paper/PP9780089 
%X Inclusions were found in protein bodies in cotyledons of seeds of each of five legume species (Acacia conferta, Cassia artemisioides, Clianthus formosus, Glycine max, Vicia faba). They were studied by a number of techniques: thin sectioning of fixed, embedded tissue; freeze-fracturing of unfixed tissue; chemical analysis of P, K, Mg and Ca content; and energy dispersive X-ray (EDX) analysis of both sections of glutaraldehyde-fixed tissue and freeze-dried tissue powders. The results of the studies presented in this paper, combined with a critical evaluation of the published research on legume protein body structure, lead us to believe that globoid crystals are a frequent occurrence in legume protein bodies. EDX analysis results indicate that electron-dense globoid crystals are rich in phytin and that phytin may also be present throughout the proteinaceous matrix portions of some legume protein bodies. 
