
%0 Journal Article
%A Crump, JA 
%A Murray, DR 
%T Proteolysis in Cotyledon Cells of <I>Phaseolus vulgaris</I> L.: Changes in Multiple Hydrolase Activities Following Germination
%J Functional Plant Biology
%D 1979/08/01
%V 6
%N 4
%P 467-474
%K 
%R https://doi.org/10.1071/PP9790467
%U https://www.publish.csiro.au/paper/PP9790467 
%X Changes in activity of several hydrolytic enzymes were measured in extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder). Aminopeptidase AP2 (phenanthroline-sensitive leucyl &#946;-naphthylamidase), N-carbobenzoxy-L- tyrosine-p-nitrophenol ester (N-CBZ-L-Tyr-pNP) hydrolase, N-benzoyl-L-tyrosineethyl ester (BTEE) hydrolase and acid phosphatase all increased in activity per cotyledon, reaching maximum values after 4, 6, 10 and 10 days respectively. By comparison, aminopeptidase AP1 (phenanthrolineinsensitive leucyl &#946;-naphthylamidase) showed maximum activity after 2 days and declined immediately. &#945;-N-benzoyl-D,L-arginine-p-nitroanilide hydrolase activity was maintained at a maximum value between days 2 and 10 before declining. Differences in maximum activity per cotyledon, initial presence or absence, and timing of both increase and decrease of N-CBZ-L-Tyr-pNP and BTEE hydrolase activities suggest that at least two enzymes contribute to these activities, and that neither activity indicates the presence of a chymotrypsin-like endopeptidase. 
