
TY  - JOUR
A1  - Crump, JA 
A1  - Murray, DR 
T1  - Proteolysis in Cotyledon Cells of <I>Phaseolus vulgaris</I> L.: Changes in Multiple Hydrolase Activities Following Germination
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1979
VL  - 6
IS  - 4
SP  - 467
EP  - 474
KW  - 
UR  - https://doi.org/10.1071/PP9790467 
N2  - Changes in activity of several hydrolytic enzymes were measured in extracts prepared from the cotyledons following germination of bean seeds (Phaseolus vulgaris cv. Hawkesbury Wonder). Aminopeptidase AP2 (phenanthroline-sensitive leucyl &#946;-naphthylamidase), N-carbobenzoxy-L- tyrosine-p-nitrophenol ester (N-CBZ-L-Tyr-pNP) hydrolase, N-benzoyl-L-tyrosineethyl ester (BTEE) hydrolase and acid phosphatase all increased in activity per cotyledon, reaching maximum values after 4, 6, 10 and 10 days respectively. By comparison, aminopeptidase AP1 (phenanthrolineinsensitive leucyl &#946;-naphthylamidase) showed maximum activity after 2 days and declined immediately. &#945;-N-benzoyl-D,L-arginine-p-nitroanilide hydrolase activity was maintained at a maximum value between days 2 and 10 before declining. Differences in maximum activity per cotyledon, initial presence or absence, and timing of both increase and decrease of N-CBZ-L-Tyr-pNP and BTEE hydrolase activities suggest that at least two enzymes contribute to these activities, and that neither activity indicates the presence of a chymotrypsin-like endopeptidase. 
ER  -

