
TY  - JOUR
A1  - Cazzonelli, Christopher Ian 
A1  - Cavallaro, Antonino Salvatore 
A1  - Botella, José Ramón 
T1  - Cloning and characterisation of ripening-induced ethylene biosynthetic genes from non-climacteric pineapple (<emph type="2">Ananas comosus</emph>) fruits
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1998
VL  - 25
IS  - 5
SP  - 513
EP  - 518
KW  - 
UR  - https://doi.org/10.1071/PP98013 
N2  - To gain a better understanding of non-climacteric fruit ripening, pineapple was used as a model system to clone and characterise two ripening-inducible cDNAs coding for two enzymes of the ethylene biosynthetic pathway, 1-aminocyclopropane-1-carboxylate (ACC) synthase (acacc-1) and 1-aminocyclo-propane- 1-carboxylate oxidase (acaco-1) respectively. Due to the extreme acidity and high polyphenolic content of pineapple fruits, a method was optimised for the extraction of high quality RNA from fruit tissue. acacc-1 is a 1080 bp ACC synthase cDNA fragment encoding 360 amino acids including 10 of the 12 amino acid residues conserved in all aminotransferases. Comparison of the deduced amino acid sequence with previously reported ACC synthases shows between 52 and 67% similarity at the protein level. Southern analysis suggests the presence of only one copy of acacc-1 in the pineapple genome. Although some acacc-1 expression is detected in green fruits, there is a 16-fold increase in the level of acacc-1 in ripe fruit tissue. acaco-1 is a partial length cDNA clone of 611 bp which codes for 203 amino acids representing approximately 66% of the ACC oxidase open reading frame. Southern analysis suggests the presence of one or two copies of the gene in the pineapple genome. Northern analysis shows the expression of acaco-1 to be highly induced in wounded leaf tissue and to a lesser extent in ripening fruit tissue. The accumulation of ACC-synthase and ACC oxidase mRNAs during pineapple fruit ripening raises new questions about the putative role of ethylene during non-climacteric fruit ripening. 
ER  -

