
%0 Journal Article
%A Milborrow, B. V. 
%A Lee, H.-S. 
%T Endogenous biosynthetic precursors of (+)-abscisic acid. VII. The 1&#8242;,4&#8242;-trans-diol is formed from ABA, it is not a precursor
%J Functional Plant Biology
%D 1998/10/26
%V 25
%N 6
%P 729-737
%K ABA, 1&#8242;,4&#8242;-trans-diol, &#91;5-3H2&#93;mevalonate, biosynthesis, avocado, glutathione reductase, NADPH.
%R https://doi.org/10.1071/PP98062
%U https://www.publish.csiro.au/paper/PP98062 
%X &#91;14C&#93;ABA fed to avocado fruit is known to be converted into the 1&#8242;,4&#8242;-trans-diol and &#91;14C&#93;1&#8242;,4&#8242;-trans-diol has been shown to be converted into ABA by several plant tissues. As a &#8216;cold trap&#8217;of trans-diol becomes labelled with 14C when &#91;14C&#93;mevalonate is converted into ABA, the trans-diol has been suggested to be the immediate precursor of ABA. This proposal has now been tested by feeding &#91;5-14C,5-3H2&#93;mevalonolactone to unripe avocado fruit and measuring the 3H :14C ratio in the ABA and in the 1&#8242;,4&#8242;-trans-diol isolated from the fruit after 16 h. Little labelled diol was present unless a &#8216;cold trap&#8217; of unlabelled 1,4-trans-diol was added with the mevalonate. One 3H atom, derived from those at C-5 of mevalonate, would be expected at C-4&#8242; of the diol, adjacent to the hydroxyl group, and another at C-5 of the side chain of the diol if the diol were a precursor of ABA (3H:14C ratio of 2:3). However, if the 4&#8242;-hydroxyl group had been oxidised to a ketone to form ABA, then the 3H atom at C-4&#8242; of the diol would have been lost and the 3H:14C ratio would be expected to be 1:3. The normalised 3H : 14C ratios of ABA and 1&#8242;,4&#8242;-trans-diol biosynthesised from &#91;14C,5-3H2&#93;mevalonate were 0.915:3 and 0.844:3 respectively and after oxidation of the diol to ABA with MnO2 the ratio was 0.869:3 i.e. there was no 3H at C-4&#8242; of the diol. These ratios are as expected for the trans-diol if it had been formed by reduction of ABA. This, and the absence of labelled diol in the fruit unless a &#8216;cold trap&#8217;was added, establishes that the 1&#8242;,4&#8242;-trans-diol is formed from ABA and it is not a precursor. The formation of the diols from newly synthesised labelled ABA in cell-free systems can be attributed to the addition to the homogenate of compounds with strong reducing potential. NADPH2+ (8.4 nmol) added to a mung bean seedling homogenate caused the reduction of (&#177;)-&#91;14C&#93;ABA (0.37 nmol, 22.5 &#181;Ci/mol) to trans-diol (1189 dpm) whereas with NADP+ only 338 dpm were present in trans-diol. Glutathione (46 nmol) caused the formation of 1214 dpm while oxidised glutathione produced 638 dpm. Less 1&#8242;,4&#8242;-cis-diol was formed. 
