
TY  - JOUR
A1  - Oram, RN 
A1  - Gillespie, JM 
A1  - Blagrove, RJ 
T1  - Genetic Effects on the Proportions of Conglutin &#947; and a Subunit of Conglutin &#946; in the Storage Proteins of <I>Lupinus angustifolius</I> L. Seeds
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1981
VL  - 8
IS  - 3
SP  - 277
EP  - 284
KW  - 
UR  - https://doi.org/10.1071/PP9810277 
N2  - The genetic variability in the proportions of conglutin &#946; and &#947; components of lupin seed storage proteins was explored in F1, F2 and F3 families generated from a cross between two genotypes of Lupinus angustifolius cv. Uniharvest and a white-seeded selection from CPI 47644. The levels of conglutin &#947; differed by a factor of two in these parents, but varied continuously in the F2 and F3 families, indicating that the parental difference is due to several genes. These parents also differed in the level of a major conglutin &#946; subunit, with a molecular weight of 30 000. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the storage globulins from individual F1, F2 and F3 seeds showed that the high level in cv. Uniharvest is controlled by an incompletely dominant allele, Bsc, at one locus, whereas the low level in CPI 47644 (white-seeded) is due to homozygosity for the recessive allele, bsc. The partial dominance of the Uniharvest gene suggests that the locus has a regulatory or processing function, so it has been designated beta subunit controller. F3 families differed significantly in the level of the conglutin &#946; subunit in Bsc/- seeds, indicating that other loci with smaller effects also influence the proportion of this subunit. The proportion of the &#946; subunit in any seed depends largely on its own genotype, and not on the genotype of the maternal plant. The proportion of conglutin &#947; in the storage proteins varied inversely with the proportion of the &#946; subunit. The implications of these results for the genetic improvement of the biological value of lupin protein are discussed. 
ER  -

