
%0 Journal Article
%A Liu, Qing 
%A Singh, Surinder P. 
%A Brubaker, Curt L. 
%A Sharp, Peter J. 
%A Green, Allan G. 
%A Marshall, D. R. 
%T Molecular cloning and expression of a cDNA encoding a microsomal <font face ="symbol">w</font>-6 fatty acid desaturase from cotton (<emph type="2">Gossypium hirsutum</emph>)
%J Functional Plant Biology
%D 1999/03/15
%V 26
%N 2
%P 101-106
%K cottonseed, Gossypium hirsutum, fatty acid, &#x03C9;-6 desaturase.
%R https://doi.org/10.1071/PP98118
%U https://www.publish.csiro.au/paper/PP98118 
%X A cDNA (ghFAD2-1) encoding a seed-specific microsomal &#x03C9;-6 desaturase was isolated from a cotton (Gossypium hirsutum L. cv. Deltapine-16) embryo cDNA library. The deduced amino acid sequence showed substantial similarity to other plant microsomal &#x03C9;-6 desaturases. Northern blot analysis indicated that the ghFAD2-1 transcript was specifically induced during embryo development and expression of the transcript could not be detected in leaves. Southern blot analysis using the coding region and 3&#8242; untranslated region of ghFAD2-1 revealed that microsomal &#x03C9;-6 desaturase in cotton is encoded by a small multigene family. There are at least two copies of ghFAD2-1 in two tetraploid cotton species (G. hirsutum L. and G. barbadense L.) and at least one copy in diploid cotton species (G. herbaceum L., G. raimondii Ulbrich and G. robinsonii Mueller). 
