
TY  - JOUR
A1  - Zhang, W.-H. 
A1  - Rengel, Z. 
T1  - Aluminium induces an increase in cytoplasmic calcium in intact wheat root apical cells
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1999
VL  - 26
IS  - 5
SP  - 401
EP  - 409
KW  - Triticum aestivum, cytosolic calcium, secondary messengers, rhizotoxicity, roots.
UR  - https://doi.org/10.1071/PP98149 
N2  - Disturbance of cytoplasmic Ca 2+ homeostasis, leading to breakdown in Ca 2+ -mediated signal transduction processes, has been suggested as a primary mechanism of aluminium (Al) rhizotoxicity in plants. To test this hypothesis, Al-related changes in cytoplasmic free activity of Ca 2+ ions (&#91;Ca 2+ &#93; c ) in root apical cells of near-isogenic wheat (Triticum aestivum L.) lines differing in Al tolerance at a single locus were examined by visualising the Ca 2+ -sensitive probe Fluo-3 with confocal laser scanning microscopy. Exposure of roots to 50 &#181;M AlCl 3 (pH 4.2) led to an increase in &#91;Ca 2+ &#93; c of root apical cells in both Al-sensitive (ES8) and Al-tolerant (ET8) wheat lines. An increase in &#91;Ca 2+ &#93; c was greater in ES8 than in ET8; after 1-h treatment with 50 &#181;M AlCl 3 an increase in &#91;Ca 2+ &#93; c was 48 and 27 % in ET8 and ES8, respectively. An increase in &#91;Ca 2+ &#93; c of ES8 roots, but not ET8 roots, was observed upon treatment with 2.6 &#181;M AlCl 3 (pH 4.5). Al-related increases in &#91;Ca 2+ &#93; c were correlated with inhibition of root growth. The Al-induced increase in the &#91;Ca 2+ &#93; c was reversible upon removing AlCl 3 . These findings provide direct evidence to support the hypothesis that Al interactions with cytoplasmic Ca 2+ are involved in the Al toxicity syndrome in plants. 
ER  -

