
%0 Journal Article
%A Gubler, F 
%A Ashford, AE 
%T Changes in Peroxidase in Isolated Aleurone Layers in Response to Gibberellic Acid
%J Functional Plant Biology
%D 1983/02/01
%V 10
%N 1
%P 87-97
%K 
%R https://doi.org/10.1071/PP9830087
%U https://www.publish.csiro.au/paper/PP9830087 
%X The effect of gibberellic acid (GA3) on peroxidase activity and isoenzyme complement was followed in isolated aleurone layers of barley (Hordeurn vulgare L.) over a 48-h period. A high level of peroxidase activity was initially present in freshly isolated aleurone layers. Disc electrophoresis resolved nine peroxidase isoenzymes. By 36 h incubation, total peroxidase activity had doubled in response to GA3 and two new peroxidase isoenzymes were observed. Peroxidase release from isolated aleurone layers was also greatly stimulated by GA3 and more isoenzymes were found in the medium. The time course of release was similar to that for &#945;-amylase. Peroxidase activity in endosperm of whole germinating grains began to increase after 2 days, peaked at 4 days and subsequently declined. 
