
%0 Journal Article
%A Millhouse, J 
%A Wiskich, JT 
%A Beevers, H 
%T Metabolite Oxidation and Transport in Mitochondria of Endosperm From Germinating Castor Bean
%J Functional Plant Biology
%D 1983/04/01
%V 10
%N 2
%P 167-177
%K 
%R https://doi.org/10.1071/PP9830167
%U https://www.publish.csiro.au/paper/PP9830167 
%X Mitochondria from germinating castor bean endosperm were purified on linear sucrose-density gradients. It is shown that succinate and malate plus glutamate are oxidized rapidly; other substrates including malate (via malic enzyme) and external NADH are oxidized slowly. It is proposed that the oxidation of NADH produced during &#946;-oxidation and the glyoxylate cycle occurs intramitochondrially via malate. Castor bean mitochondria are relatively impermeable to oxaloacetate and a malate-aspartate type shuttle is required. Metabolite transport and exchange properties support the operation of malatein (or succinatein)/2-oxoglutarateout and glutamatein/aspartateout shuttles. Not all the exchange systems were reversible. The results support proposed schemes for metabolite transfer between glyoxysomes and mitochondria of castor bean endosperm during germination. 
