
TY  - JOUR
A1  - Larkin, PJ 
A1  - Taylor, BH 
A1  - Gersmann, M 
A1  - Brettell, RIS 
T1  - Direct Gene Transfer to Protoplasts
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1990
VL  - 17
IS  - 3
SP  - 291
EP  - 302
KW  - 
UR  - https://doi.org/10.1071/PP9900291 
N2  - Direct transfer of genes to protoplasts is considered, using electroporation and polyethylene-glycol-induced plasmid uptake. Both transient expression and stable transformation are discussed, with data and comparisons from the following protoplast systems: Nicotiana plumbaginifolia suspension culture, Nicotiana tabacum leaf, Medicago sativa leaf and Triticum monococcum suspension culture. 
ER  -

