
TY  - JOUR
A1  - Upadhyaya, Narayana M. 
A1  - Surin, Brian 
A1  - Ramm, Kerrie 
A1  - Gaudron, Judy 
A1  - Schünmann, Petra H. D. 
A1  - Taylor, William 
A1  - Waterhouse, Peter M. 
A1  - Wang, Ming-Bo 
T1  - <emph type="2">Agrobacterium</emph>-mediated transformation of Australian rice cultivars Jarrah and Amaroo using modified promoters and selectable markers
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2000
VL  - 27
IS  - 3
SP  - 201
EP  - 210
KW  - Agrobacterium, Australian rice, GFP, Oryza sativa, promoters, selectable markers, transformation.
UR  - https://doi.org/10.1071/PP99078 
N2  - We report the first successfulAgrobacterium-mediated transformation of Australian elite rice cultivars, Jarrah and Amaroo, using binary vectors with our improved promoters and selectable markers. Calli derived from mature embryos were used as target tissues. The binary vectors contained hph(encoding hygromycin resistance) or bar (encoding herbicide resistance) as the selectable marker gene and uidA (gus) or sgfpS65T as the reporter gene driven by different promoters. Use of Agrobacterium strain AGL1 carrying derivatives of an improved binary vector pWBVec8, wherein the CaMV35S driven hph gene is interrupted by the castor bean catalase 1 intron, produced a 4- fold higher number of independent transgenic lines compared to that produced with the use of strain EHA101 car-rying the binary vector pIG121-Hm wherein the CaMV35S driven hph is intronless. The Ubiquitin promoter produced 30-fold higher &szlig;-glucuronidase (GUS) activity (derivatives of binary vector pWBVec8) in transgenic plants than the CaMV35S promoter (pIG121-Hm). The two modified SCSV promoters produced GUS activity com-parable to that produced by the Ubiquitin promoter. Progeny analysis (R1) for hygromycin resistance and GUS activ-ity with selected lines showed both Mendelian and non-Mendelian segregation. Lines showing very high levels of GUS activity in T0 showed a reduced level of GUS activity in their T1 progeny, while lines with moderate levels of GUS activity showed increased levels in T1 progeny. Stable heritable green fluorescent protein (GFP) expression was also observed in few transgenic plants produced with the binary vector pTO134 which had the CaMV35S promoter-driven selectable marker gene bar and a modified CaMV35S promoter-driven reporter gene sgfpS65T. 
ER  -

