
%0 Journal Article
%A Di Martino Rigano, Vittoria 
%A Vona, Vincenza 
%A Carfagna, Simona 
%A Esposito, Sergio 
%A Carillo, Petronia 
%A Rigano, Carmelo 
%T Effects of sulfate-starvation and re-supply on growth, NH<emph type="8">4</emph><emph type="7">+</emph> uptake and starch metabolism in <emph type="2">Chlorella sorokiniana</emph>
%J Functional Plant Biology
%D 2000/05/10
%V 27
%N 4
%P 335-342
%K algal growth, algal respiration, sulfurassimilation, Chlorella sorokiniana, photosynthesis.
%R https://doi.org/10.1071/PP99090
%U https://www.publish.csiro.au/paper/PP99090 
%X Chlorella sorokinianaShihira &#38; Krauss, strain 211/8K resuspended in a illuminated mediumshowed continuous growth, and concomitantly NH4 +in the medium depleted at a constant rate. Upon sulfate removal, i) growthdeclined, ceasing within 5 h; ii) NH4 + depletionbecame almost undetectable over 2 h; and iii) photosynthetic capacity (Pc) wasreduced over 24 h by 80% in the light, but was reduced by only30% in darkness. Over 24 h of S starvation the chlorophyll content ofilluminated cells decreased by 50%, whereas that of darkened cellsdecreased by only 10%. Sulfate-deprivation over 24 h resulted in anoticeable increase in the starch content of the cell and a net increase inthe pools of glutamate, glutamine, serine and asparagine. Cysteine content, incontrast, was decreased. Sulfate addition to cells starved of S for 24 hprovoked a sudden time-dependent increase in Pc and in an immediate renewal ofgrowth in light but not in darkness. Sulphate supply also caused a suddenenhancement of respiratory oxygen consumption and a prompt degradation ofstarch. Starch was degraded at a higher rate in illuminated than in darkenedcells. After the S addition, depletion of NH4 + inthe medium occurred at slowly increasing rates; amino acids decreasednoticeably over 4 h, where in contrast, cysteine increased noticeably. 
