
TY  - JOUR
A1  - Di Martino Rigano, Vittoria 
A1  - Vona, Vincenza 
A1  - Carfagna, Simona 
A1  - Esposito, Sergio 
A1  - Carillo, Petronia 
A1  - Rigano, Carmelo 
T1  - Effects of sulfate-starvation and re-supply on growth, NH<emph type="8">4</emph><emph type="7">+</emph> uptake and starch metabolism in <emph type="2">Chlorella sorokiniana</emph>
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 2000
VL  - 27
IS  - 4
SP  - 335
EP  - 342
KW  - algal growth, algal respiration, sulfurassimilation, Chlorella sorokiniana, photosynthesis.
UR  - https://doi.org/10.1071/PP99090 
N2  - Chlorella sorokinianaShihira &#38; Krauss, strain 211/8K resuspended in a illuminated mediumshowed continuous growth, and concomitantly NH4 +in the medium depleted at a constant rate. Upon sulfate removal, i) growthdeclined, ceasing within 5 h; ii) NH4 + depletionbecame almost undetectable over 2 h; and iii) photosynthetic capacity (Pc) wasreduced over 24 h by 80% in the light, but was reduced by only30% in darkness. Over 24 h of S starvation the chlorophyll content ofilluminated cells decreased by 50%, whereas that of darkened cellsdecreased by only 10%. Sulfate-deprivation over 24 h resulted in anoticeable increase in the starch content of the cell and a net increase inthe pools of glutamate, glutamine, serine and asparagine. Cysteine content, incontrast, was decreased. Sulfate addition to cells starved of S for 24 hprovoked a sudden time-dependent increase in Pc and in an immediate renewal ofgrowth in light but not in darkness. Sulphate supply also caused a suddenenhancement of respiratory oxygen consumption and a prompt degradation ofstarch. Starch was degraded at a higher rate in illuminated than in darkenedcells. After the S addition, depletion of NH4 + inthe medium occurred at slowly increasing rates; amino acids decreasednoticeably over 4 h, where in contrast, cysteine increased noticeably. 
ER  -

