
%0 Journal Article
%A Makino, Amane 
%A Harada, Masayo 
%A Kaneko, Kentaro 
%A Mae, Tadahiko 
%A Shimada, Takiko 
%A Yamamoto, Naoki 
%T Whole-plant growth and N allocation in transgenic rice plants with decreased content of ribulose-1,5-bisphosphate carboxylase under different CO<sub>2</sub> partial pressures
%J Functional Plant Biology
%D 2000/01/24
%V 27
%N 1
%P 1-12
%K biomass, elevated CO2, nitrogen allocation, Oryza sativa L, photosynthesis, ribulose-1,5- bisphosphate carboxylase/oxygenase
%R https://doi.org/10.1071/PP99094
%U https://www.publish.csiro.au/paper/PP99094 
%X Growth of transgenic rice (Oryza sativa L.) with an antisense gene to the small subunit of Rubisco was analysed under 36 and 100 Pa CO2 during a 14-h photoperiod (1000 mol quanta m&#8211;2 s&#8211;1). Two lines of the antisense plants were used; one with 65% wild-type Rubisco and the other with 40% wild-type Rubisco. The plants were grown hydroponically for 70 d. The final biomass of the antisense plants grown in 36 Pa CO2 was much smaller than that of the wild-type plant. However, several compen-sation phenomena were found in the antisense plants. Increased biomass allocation to leaf blades and preferential N investment in leaf blades were observed. Leaf senescence was also delayed. Elevated CO2 levels up to 100 Pa caused the antisense plants to achieve a size similar to that of the wild-type plant. However, although the antisense plant with 65% wild-type Rubisco was selected as a plant with optimal Rubisco content for CO2 -saturated photosynthesis, its final biomass was not greater than that of the wild-type plant. This may have been caused by a relatively strong Rubisco-antisense effect during the early stage of growth (21&#8211;42 d). N-use efficiency for growth after d 42 was greater in the selected antisense plant. Thus, improvement of N-use efficiency at the level of a single leaf did not necessarily lead to greater production of biomass at the whole-plant level. 
