
%0 Journal Article
%A Nomura, Mika 
%A Sentoku, Naoki 
%A Tajima, Shigeyuki 
%A Matsuoka, Makoto 
%T Expression patterns of cytoplasmic pyruvate, orthophosphate dikinase of rice (C<emph type="8">3</emph>) and maize (C<emph type="8">4</emph>) in a C<emph type="8">3</emph> plant, rice
%J Functional Plant Biology
%D 2000/05/10
%V 27
%N 4
%P 343-347
%K C4 plant, evolution, pyruvate, orthophosphate dikinase, maize, rice.
%R https://doi.org/10.1071/PP99190
%U https://www.publish.csiro.au/paper/PP99190 
%X Two types of mRNAs are transcribed from the C4-type pyruvate, orthophosphate dikinase gene (Pdk) with different sizes, which encode chloroplastic and cytoplasmic forms of the enzyme. The two transcripts are pro duced by two independent promoters and this unusual dual promoter system is also found in the C4-like Pdk gene of the C3 plant, rice. In order to elucidate the expression pattern of the cytoplasmic transcript from the maize C4-type and rice C4-like Pdk genes, we have produced chimeric constructs with the &szlig;-glucuronidase (GUS) reporter gene under the control of the cytoplasmic promoters and introduced the constructs into rice. Both cytoplasmic promoters directed GUS expression in non-photosynthetic organs, such as endosperm and roots, in transgenic rice plants, while expression was low in photosynthetic organs. These results indicate that the organ-specific localization of the cyto-plasmic enzyme is similar in C3 and C4 plants. The results also suggest the possibility that the cytoplasmic enzyme has a similar function(s) in non-photosynthetic organs both in C3 and C4 plants. 
