
%0 Journal Article
%A Botha, AM 
%A Botha, FC 
%T Effect of the Radicle, and Hormones on the Subunit Composition and Molecular Form of Pyrophosphate-Dependent Phosphofructokinase in the Cotyledons of <I>Citrullus lanatus</I>
%J Functional Plant Biology
%D 1993/06/01
%V 20
%N 3
%P 265-273
%K 
%R https://doi.org/10.1071/PP9930265
%U https://www.publish.csiro.au/paper/PP9930265 
%X After initiation of radicle elongation, the pyrophosphate dependent phosphofructokinase (PFP) activity increases in the cotyledons of Citrullus lanatus. A selective increase of the &#946;-subunit and a constant level of the &#945;-subunit is apparent in the cotyledons during early seedling establishment. The radicle plays an important role in the control of the level of PFP activity and &#946;-subunit content in the cotyledons, as removal during the incubation prevented the increase in PFP activity. Radicle removal also largely restricted the increase in specific &#946;-subunit content, observed in the intact embryos. Application of kinetin (0.5 &#956;M) or 2-chlorophosphonic acid (0.1 &#956;M ethrel) to isolated embryos replaces the effect of the radicle. Application of ethrel to isolated embryos increased the specific &#946;-subunit, while the &#945;-subunit content remained constant. Exogenous kinetin resulted in a substantial increase in the specific &#946;-subunit content, while the &#945;-subunit content declined. The PFP isoenzyme ratio differs between the hormonal treatments and the control tissue, probably as a result of their differential effect on the level of the PFP subunits. 
