
TY  - JOUR
A1  - Botha, AM 
A1  - Botha, FC 
T1  - Effect of the Radicle, and Hormones on the Subunit Composition and Molecular Form of Pyrophosphate-Dependent Phosphofructokinase in the Cotyledons of <I>Citrullus lanatus</I>
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1993
VL  - 20
IS  - 3
SP  - 265
EP  - 273
KW  - 
UR  - https://doi.org/10.1071/PP9930265 
N2  - After initiation of radicle elongation, the pyrophosphate dependent phosphofructokinase (PFP) activity increases in the cotyledons of Citrullus lanatus. A selective increase of the &#946;-subunit and a constant level of the &#945;-subunit is apparent in the cotyledons during early seedling establishment. The radicle plays an important role in the control of the level of PFP activity and &#946;-subunit content in the cotyledons, as removal during the incubation prevented the increase in PFP activity. Radicle removal also largely restricted the increase in specific &#946;-subunit content, observed in the intact embryos. Application of kinetin (0.5 &#956;M) or 2-chlorophosphonic acid (0.1 &#956;M ethrel) to isolated embryos replaces the effect of the radicle. Application of ethrel to isolated embryos increased the specific &#946;-subunit, while the &#945;-subunit content remained constant. Exogenous kinetin resulted in a substantial increase in the specific &#946;-subunit content, while the &#945;-subunit content declined. The PFP isoenzyme ratio differs between the hormonal treatments and the control tissue, probably as a result of their differential effect on the level of the PFP subunits. 
ER  -

