
%0 Journal Article
%A Livingstone, DM 
%A Birch, RG 
%T Plant Regeneration and Microprojectile-Mediated Gene Transfer in Embryonic Leaflets of Peanut (<I>Arachis hypogaea</I> L.)
%J Functional Plant Biology
%D 1995/08/01
%V 22
%N 4
%P 585-591
%K 
%R https://doi.org/10.1071/PP9950585
%U https://www.publish.csiro.au/paper/PP9950585 
%X Excised leaflets from mature embryos of peanut cultivars Robut, Gajah, McCubbin and NC-7 produced multiple shoot primordia via organogenesis on Murashige and Skoog medium supplemented with 3 mg L-1 BAP and 1 mg L-1 NAA. Plants were regenerated by elongation of shoot primordia on transfer to MS medium supplemented with 5 mg L-1 BAP. The regeneration frequency of five plants per embryonic leaflet was 10-40-fold higher than previously reported from seedling leaflets. Microprojectile bombardment parameters were optimised for high transient expression rates of the &#946;-glucuronidase (uidA or gus) reporter gene in peanut embryos (> 2000 cells per bombardment) and in excised embryonic leaflets (> 1200 cells per bombardment). The firefly luciferase reporter gene (luc) allowed repeated non-toxic assays, revealing the transition from hundreds of transiently expressing cells soon after bombardment, to a few stably expressing regions in callus grown without selection for 8 weeks after bombardment of embryonic leaflets. 
