
%0 Journal Article
%A Chapple, RM 
%A Chaudhury, AM 
%A Blomer, KC 
%A Farrell, LB 
%A Dennis, ES 
%T Construction of a YAC Contig of 2 Megabases Around the MS1 Gene in <I>Arabidopsis thaliana</I>
%J Functional Plant Biology
%D 1996/08/01
%V 23
%N 4
%P 453-465
%K 
%R https://doi.org/10.1071/PP9960453
%U https://www.publish.csiro.au/paper/PP9960453 
%X The ms1 mutation of Arabidopsis thaliana causes male sterility by preventing the development of normal microspores in the developing anther. The gene is located on a region of chromosome 5 containing the RFLP markers g4111, g4560 and g21503. Using yeast artificial chromosome (YAC) libraries, we have constructed a contig of 38 YACs spanning approximately 2.1 megabases (approximately 2% of the genome) around MS1 and redefined the order of these RFLP markers. Chimeric YACs and repetitive DNA caused problems in chromosome 'walking'. A method for cloning YAC right ends by plasmid rescue was applied to arabidopsis. One YAC end contained a portion of the A. thaliana sucrose synthase gene ASUSI, hence locating this gene on chromosome 5 near MS1. Using recombinant plant populations containing ms1 and flanking markers, MS1 was localised to a 200 kb region within the YAC contig. In this contig the relationship between physical and genetic distance varied from less than 100 kb to 720 kb per centimorgan. 
