
TY  - JOUR
A1  - Stone, PJ 
A1  - Nicolas, ME 
A1  - Wardlaw, IF 
T1  - The Influence of Recovery Treatment on the Effects of a Brief Heat Shock on Wheat. II. Fractional Protein Accumulation During Grain Growth
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1996
VL  - 23
IS  - 5
SP  - 605
EP  - 616
KW  - 
UR  - https://doi.org/10.1071/PP9960605 
N2  - In this study, we have sought to identify the nature of the response to both moderately high (25-32ºC) and very high (>32ºC) temperatures by examining their interactive effects on the accumulation of functionally important proteins during grain-filling. In particular, we wished to determine if the deleterious effects of very high temperature could be alleviated by subsequently cool conditions. To this end, wheat cv. Oxley was exposed to either 21/16 or 40/16ºC (day/night) from 15 to 19 days after anthesis and subsequently grown under one of three temperature regimes until maturity: 21/16 (control), 27/22 or 30/25ºC. Grain samples were taken throughout grain growth and analysed for protein content and composition. Wheat proteins were separated and quantified as albumin,globulin, monomer, SDS-soluble polymer and SDS-insoluble polymer using size-exclusion high-performance liquid chromatography.     The various protein fractions responded differentially and usually additively to moderately high and very high temperatures during grain-filling. Cool post-shock conditions did not alleviate the effects of very high temperature on grain protein composition. Heat treatments reduced polymer:monomer ratio because the accumulation of monomer was reduced less than that of polymer. 
ER  -

