
%0 Journal Article
%A Martinelli, Laurie 
%T <emph type="2">Australian Journal of Plant Physiology</emph>: Editorial report
%J Functional Plant Biology
%D 1998/03/30
%V 25
%N 1
%P I-Ii
%K Arabidopsis, T-DNA tagging, in planta transformation, flowering-time gene, GIGANTEA.
%R https://doi.org/10.1071/PPV25N1_ED
%U https://www.publish.csiro.au/paper/PPV25N1_ED 
%X Gene tagging with insertional mutagens greatly facilitates the isolation of novel genes. A new collection of Arabidopsis T-DNA tag insertion lines (n=2165) was generated by in planta transformation. Whole plants were vacuum-infiltrated in a suspension of Agrobacterium carrying the pGKB5 tagging vector. The efficiency of transformation increased with addition of the surfactant Silwet L-77 (0.005% v/v) to the Agrobacterium suspension. Visual screens of the T-DNA lines identified two mutants with floral defects. Allelism tests suggested that a mutation in the GIGANTEA gene was responsible for the late-flowering phenotype of one of the mutants. Linkage analysis indicated that the GIGANTEA gene was tagged in this mutant. 
