
TY  - JOUR
A1  - Martinelli, Laurie 
T1  - <emph type="2">Australian Journal of Plant Physiology</emph>: Editorial report
JF  - Functional Plant Biology
JO  - Functional Plant Biol.
Y1  - 1998
VL  - 25
IS  - 1
SP  - I
EP  - Ii
KW  - Arabidopsis, T-DNA tagging, in planta transformation, flowering-time gene, GIGANTEA.
UR  - https://doi.org/10.1071/PPV25N1_ED 
N2  - Gene tagging with insertional mutagens greatly facilitates the isolation of novel genes. A new collection of Arabidopsis T-DNA tag insertion lines (n=2165) was generated by in planta transformation. Whole plants were vacuum-infiltrated in a suspension of Agrobacterium carrying the pGKB5 tagging vector. The efficiency of transformation increased with addition of the surfactant Silwet L-77 (0.005% v/v) to the Agrobacterium suspension. Visual screens of the T-DNA lines identified two mutants with floral defects. Allelism tests suggested that a mutation in the GIGANTEA gene was responsible for the late-flowering phenotype of one of the mutants. Linkage analysis indicated that the GIGANTEA gene was tagged in this mutant. 
ER  -

