
TY  - JOUR
A1  - Wells, Rufus M. G. 
A1  - Baldwin, John 
A1  - Seymour, Roger S. 
T1  - Low concentrations of methaemoglobin in marine fishes of the Great Barrier Reef, Australia
JF  - Marine and Freshwater Research
JO  - Mar. Freshwater Res.
Y1  - 1997
VL  - 48
IS  - 4
SP  - 303
EP  - 309
KW  - 
UR  - https://doi.org/10.1071/MF97024 
N2  - Concentrations of methaemoglobin (the oxidized non-functional ferric form of haemoglobin) in the blood of marine fish are poorly documented. Although high concentrations have been reported for fish maintained in captivity, baseline values for wild populations are unknown. Two techniques, the cyanide derivative method and the multiple wavelength method, were used to determine methaemoglobin concentrations in blood samples from 25 species of marine teleosts and elasmobranchs captured on the Australian Great Barrier Reef. Although methaemoglobin generally accounted for less than 2% of total haemoglobin, systematic errors occurred when these two standard methods, developed for mammalian blood, were applied to the blood of some fish species. Most problems arose from reactions of various blood components with the reagents used in the cyanide derivative method. Consequently, the multiple wavelength method generally was more reliable for estimating methaemoglobin in the blood of marine fish. The low methaemoglobin concentrations in fish studied on the Great Barrier Reef indicate high water quality and healthy physiological condition. 
ER  -

