
TY  - JOUR
A1  - Morehead, D. T. 
T1  - Effect of capture, confinement and repeated sampling on plasma steroid concentrations and oocyte size in female striped trumpeter <emph type="2">Latris lineata</emph> (Latrididae)
JF  - Marine and Freshwater Research
JO  - Mar. Freshwater Res.
Y1  - 1998
VL  - 49
IS  - 5
SP  - 373
EP  - 377
KW  - cortisol, stress.
UR  - https://doi.org/10.1071/MF97245 
N2  - Wild female striped trumpeter were caught during the spawning season to examine the effect of capture, confinement and repeated handling on reproductive function. Blood and ovarian samples were obtained from 5 females within 10 minutes of capture and from a pool of female fish (n = 17), including the original 5, that were sampled regularly from Day 2 to Day 13 after capture. Plasma concentrations of cortisol, testosterone (T), 17&#946;-oestradiol (E2) and 17,20&#946;-dihydroxy-4-pregnen-3-one (17,20&#946;P) were measured by radioimmunoassay. Four of the 17 fish ovulated and a small volume of eggs was obtained by hand stripping. Mean oocyte size did not change significantly, although there was a general trend toward decreasing oocyte diameters over time. Plasma concentrations of T and E2 decreased significantly after capture, and remained low thereafter. Concentrations of 17,20&#946;P remained unchanged apart from a decrease on Day 13. Cortisol concentrations significantly increased after capture and remained elevated until Day 5, with subsequent elevations on Days 7 and 9. These results suggest that the stress of capture, confinement and repeated handling inhibits the ovarian production of T and E2 but not 17,20&#946;P and arrests oocyte development at all but the most advanced stages. 
ER  -

