
TY  - JOUR
A1  - Innes, B. H. 
A1  - Grewe, P. M. 
A1  - Ward, R. D. 
T1  - PCR-based genetic identification of marlin and other billfish
JF  - Marine and Freshwater Research
JO  - Mar. Freshwater Res.
Y1  - 1998
VL  - 49
IS  - 5
SP  - 383
EP  - 388
KW  - 
UR  - https://doi.org/10.1071/MF98007 
N2  - A genetic test was developed for the identification of the six species of billfish found in Australian waters (black marlin, Indo&#8211;Pacific blue marlin, striped marlin, Indo&#8211;Pacific sailfish, shortbill spearfish and broadbill swordfish). The test was based on the PCR&#8211;RFLP analysis of a 1400 bp region of the mitochondrial DNA molecule, the d-loop, using four restriction enzymes (Hinf I, Rsa I and Sau3A I andTaq I). A total of 33 composite haplotypes were observed among 160 fish; all were species-specific. Three of the species&#8212;black marlin, striped marlin and broadbill swordfish&#8212;showed sufficient intra-specific variation to be useful in population structure analyses. 
ER  -

