
TY  - JOUR
A1  - Jawerbaum, Alicia 
A1  - Gonzalez, Elida T. 
A1  - Faletti, Alicia 
A1  - Novaro, Virginia 
A1  - Gimeno, Martha A. F. 
T1  - Nitric oxide mediates human chorionic gonadotrophin-induced prostaglandin E generation in rat oocyte&#8211;cumulus complexes
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1997
VL  - 9
IS  - 4
SP  - 391
EP  - 394
KW  - 
UR  - https://doi.org/10.1071/R96043 
N2  - To determine whether nitric oxide (NO) generation mediates human chorionic gonadotrophin (hCG)-induced prostaglandin E (PGE) secretion by oocyte&#8211;cumulus complexes (OCC), the secretion of PGE by cultured rat OCC in the presence of NO donors and NO synthase (NOS) inhibitors was characterized. NO donors (sodium nitroprusside and 3-morpholino-sydnonimine- hydrochloride) increased PGE accumulation in OCC to values similar to those obtained in the presence of hCG. The three NOS inhibitors tested (N G -nitro-L-arginine methyl ester, NG -monomethyl-L-arginine and aminoguanidine) prevented the hCG-induced PGE accumulation in cultured OCC. This effect appears to be specific since D-enantiomers NG -nitro-D-arginine methyl ester and NG -monomethyl-D-arginine had no effect. The present results suggest that NO mediates the hCG-induced accumulation of PGE in rat OCC, a process which may occur in vivo in preovulatory follicles prior to ovulation. 
ER  -

