
%0 Journal Article
%A Cha, S. K. 
%A Kim, N.-H. 
%A Lee, S. M. 
%A Baik, C. S. 
%A Lee, H. T. 
%A Chung, K. S. 
%T Effect of cytochalasin B and cycloheximide on the activation rate, chromosome constituent and <emph type="2">in vitro</emph> development of porcine oocytes following parthenogenetic stimulation
%J Reproduction, Fertility and Development
%D 1997/11/14
%V 9
%N 4
%P 441-446
%K pig, parthenogenesis, ploidy.
%R https://doi.org/10.1071/R96078
%U https://www.publish.csiro.au/paper/R96078 
%X Activation rate, chromosome constituent and developmental pattern of porcine oocytes was examined in the presence and absence of cytochalasin B and cycloheximide following parthenogenetic stimulation. Treatment with cycloheximide after ethanol or Ca2+ ionophore treatment increased the incidence of activation. The percentage of oocytes with two or more female pronuclei was higher (P &#60; 0&#183; 05) in oocytes treated with cytochalasin B than in control or cycloheximide-treated oocytes. Treatment with both electrical stimulation and cytochalasin B increased the incidence of diploid chromosome spreads, and accelerated development to the morula and blastocyst stage compared with the control and cycloheximide-treated groups, suggesting a role of ploidy in the development of parthenote. 
