
TY  - JOUR
A1  - Cha, S. K. 
A1  - Kim, N.-H. 
A1  - Lee, S. M. 
A1  - Baik, C. S. 
A1  - Lee, H. T. 
A1  - Chung, K. S. 
T1  - Effect of cytochalasin B and cycloheximide on the activation rate, chromosome constituent and <emph type="2">in vitro</emph> development of porcine oocytes following parthenogenetic stimulation
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1997
VL  - 9
IS  - 4
SP  - 441
EP  - 446
KW  - pig, parthenogenesis, ploidy.
UR  - https://doi.org/10.1071/R96078 
N2  - Activation rate, chromosome constituent and developmental pattern of porcine oocytes was examined in the presence and absence of cytochalasin B and cycloheximide following parthenogenetic stimulation. Treatment with cycloheximide after ethanol or Ca2+ ionophore treatment increased the incidence of activation. The percentage of oocytes with two or more female pronuclei was higher (P &#60; 0&#183; 05) in oocytes treated with cytochalasin B than in control or cycloheximide-treated oocytes. Treatment with both electrical stimulation and cytochalasin B increased the incidence of diploid chromosome spreads, and accelerated development to the morula and blastocyst stage compared with the control and cycloheximide-treated groups, suggesting a role of ploidy in the development of parthenote. 
ER  -

