
TY  - JOUR
A1  - Nikolova, Diana B. 
A1  - Martinova, Yordanka S. 
A1  - Seidensticker, Martin 
A1  - Bellvé, Anthony R. 
T1  - Leukaemia inhibitory factor stimulates proliferation of prospermatogonial stem cells
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1998
VL  - 9
IS  - 7
SP  - 717
EP  - 722
KW  - seminiferous growth factor (SGF).
UR  - https://doi.org/10.1071/R96116 
N2  - The effect of leukaemia inhibitory factor (LIF) on the proliferation of prospermatogonial stem cells was testedin vitro. Pieces of 3-day-old rat testis were cultured in the presence of a range of doses of LIF alone or in combination with 1 ng mL -1 seminiferous growth factor (SGF). Stimulation of the proliferative activity of quiescent prospermatogonia was detected immunocytochemically with a cell proliferation kit. After 24 h culture, LIF signicantly increased the percentage of labelled prospermatogonia, with the peak of activity at 20 pg mL -1 . The combination of LIF and SGF resulted in a decrease in DNA synthetic activity of the germ cells. Hence, LIF and SGF play a role in local regulation at the onset of spermatogenesis in the rat testis. 
ER  -

