
TY  - JOUR
A1  - Watanabe, Hiroko 
A1  - Miyamoto, Akio 
A1  - Fukui, Yutaka 
T1  - A competitive enzyme immunoassay for follicle-stimulating hormone in ovine plasma using biotin-streptavidin amplification
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1998
VL  - 9
IS  - 6
SP  - 597
EP  - 602
KW  - EIA, ovine FSH.
UR  - https://doi.org/10.1071/R97037 
N2  - To determine follicle-stimulating hormone (FSH) concentration in ovine plasma, a second-antibody enzyme immunoassay using biotin-streptavidin amplication (BS-EIA) was developed and was validated by comparison with a routine radioimmunoassay (RIA). Ovine FSH was labelled with D-biotinyl-b.epsi;-aminocaproic acid-N-hydroxysuccinimide ester (biotin-7-NHS). The minimum and maximum detection limits were 0&#183;40 ng mL-1 and 25&#183;0 ng mL-1 respectively in the BS-EIA. Plasma samples from three superovulated ewes were measured for FSH by both BS-EIA and RIA. Correlation between values obtained by BS-EIA and RIA was 0&#183; 86 (n = 106), and the linear regression had a slope of 0&#183;88 and an intercept of -0&#183;07 (R2 = 0&#183;74). The baseline of BS-EIA was slightly lower than that of RIA (0&#183;69 ng mL-1v. 1&#183;10 ng mL-1). The intra-assay and inter-assay coefcients of variation in BS-EIA were 13&#183;8% and 16&#183;7% (n = 106) respectively. The recovery rates were 104&#183; 8 2&#183;7% (mean&#177;s.e.m., n = 14). Changes in plasma FSH concentration from superovulated ewes evaluated by BS-EIA corresponded well with the results of routine RIA. 
ER  -

