
%0 Journal Article
%A Jones, A. R. 
%T Metabolic activity of hypotonically treated mature boar spermatozoa
%J Reproduction, Fertility and Development
%D 1998/03/23
%V 9
%N 6
%P 583-586
%K mitochondria, glycerol 3-phosphate, lactate.
%R https://doi.org/10.1071/R97048
%U https://www.publish.csiro.au/paper/R97048 
%X Treatment of washed boar sperm with hypotonic phosphate buffer removed the acrosome, disrupted the cytoplasmic membrane and almost completely separated the heads from the mid piece-tail segment. As assessed by oxygen uptake studies and their ability to oxidize14C-labelled substrates to 14CO2, hypotonically-treated cells exhibit low glycolytic activity yet mitochondrial activity remains high. Both lactate and glycerol 3-phosphate underwent oxidation and these substrates continued to be metabolized by this preparation which had been stored for up to 10 days at 4&#176;C. Such preparations may be of assistance in the investigation of the biochemistry of boar sperm mitochondria. 
