
TY  - JOUR
A1  - Jones, A. R. 
T1  - Metabolic activity of hypotonically treated mature boar spermatozoa
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1998
VL  - 9
IS  - 6
SP  - 583
EP  - 586
KW  - mitochondria, glycerol 3-phosphate, lactate.
UR  - https://doi.org/10.1071/R97048 
N2  - Treatment of washed boar sperm with hypotonic phosphate buffer removed the acrosome, disrupted the cytoplasmic membrane and almost completely separated the heads from the mid piece-tail segment. As assessed by oxygen uptake studies and their ability to oxidize14C-labelled substrates to 14CO2, hypotonically-treated cells exhibit low glycolytic activity yet mitochondrial activity remains high. Both lactate and glycerol 3-phosphate underwent oxidation and these substrates continued to be metabolized by this preparation which had been stored for up to 10 days at 4&#176;C. Such preparations may be of assistance in the investigation of the biochemistry of boar sperm mitochondria. 
ER  -

