
TY  - JOUR
A1  - Ain, Rupasri 
A1  - Seshagiri, P. B. 
T1  - Micromolar concentration of pentoxifylline improves development <emph type="2">in vitro</emph> of hamster 8-cell embryos: conrmation of biological viability by embryo transfer
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1998
VL  - 9
IS  - 7
SP  - 697
EP  - 702
KW  - blastocyst development, hamster, embryo viability.
UR  - https://doi.org/10.1071/R97052 
N2  - The in&szlig;uence of the sperm motility stimulant pentoxifylline (PF) on preimplantation embryo development in hamsters was evaluated. Eight-cell embryos were cultured in hamster embryo culture medium (HECM)-2, with or without PF (0&#183; 0233&#183;6 mM). There was 90%, 37% and 29% inhibition of blastocyst development by 3&#183;6 (used for human sperm), 0&#183;9 and 0 &#183;45 mM PF, respectively. However, 23 &#181;M PF (exposed to hamster oocytes during IVF) signicantly (P &#60; 0&#183;05) improved blastocyst development (63&#183; 6% v. 51&#183; 8%); morulae development was, however, not curtailed by 0&#183;45 mM or 0&#183;9 mM PF (51&#183;8%&#177;6&#183;0 or 50&#183;5%&#177;11&#183;3, respectively). Post-implantation viability of PF-treated embryos was assessed by embryo transfer; 43% of 80 PF-treated embryos implanted compared with 40% of 79 control embryos. Of the 9 recipients, 6 females delivered pups (19, i.e. 16% of transferred embryos or 53% of implanted embryos). These data show that in hamsters, continuous presence of PF at 0&#183;45-3&#183;6 mM is detrimental to 8-cell embryo development whereas 23 &#181;M PF improves the development of embryos to viable blastocysts which produce live offspring. 
ER  -

