
TY  - JOUR
A1  - Asano, H. 
A1  - Shearman, K. 
A1  - Darnel, A. 
A1  - Richardson, B. S. 
A1  - Yang, K. 
T1  - Effects of sustained hypoxaemia with 72 hours recovery on 11&#946;-hydroxysteroid dehydrogenase types 1 and 2 gene expression in near-term fetal sheep
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1998
VL  - 9
IS  - 8
SP  - 755
EP  - 762
KW  - 11&#946;-HSD1 and 2; mRNA; enzyme activity; glucocorticoid bioavailability; metabolic acidosis
UR  - https://doi.org/10.1071/R97070 
N2  - The study examined the effects of 8 h sustained hypoxaemia, with 72 h recovery, on the expression of 11&#946;-hydroxysteroid dehydrogenase (11b-HSD) types 1 and 2 in near-term fetal sheep. Placental tissue and fetal liver and kidney were collected at Days 135&#8211;138 gestation 72 h after 8 h sustained hypoxaemia induced by lowering maternal inspired oxygen with (n= 9) and without (n = 6) metabolic acidosis or after 8 h normoxia (n = 6). In hypoxic fetuses with metabolic acidosis, a significant increase in the level of 11&#946;-HSD2 mRNA in the kidney compared with controls was correlated significantly with degree of associated fetal acidaemia, but there were no corresponding increases in the tissue level of 11&#946;-HSD2 activity. Hence, a time lag may exist between the mRNA and activity. Alternatively, the translation of 11&#946;-HSD2 mRNA may be inhibited. In contrast, levels of 11&#946;-HSD1 mRNA in the placenta and fetal liver were unchanged 72 h after sustained hypoxaemia. These results indicate that sustained fetal hypoxaemia with metabolic acidosis selectively up-regulates 11&#946;-HSD2 mRNA expression in the near-term fetal sheep kidney. This may be a re-bound effect at 72 h following an initial down-regulation as observed in a previous study. 
ER  -

