
%0 Journal Article
%A Chauhan, M. S. 
%A Singla, S. K. 
%A Palta, P. 
%A Manik, R. S. 
%A Madan, M. L. 
%T <emph type="2">In vitro</emph> maturation and fertilization, and subsequent development of buffalo (<emph type="2">Bubalus bubalis</emph>) embryos: effects of oocyte quality and type of serum
%J Reproduction, Fertility and Development
%D 1998/10/09
%V 10
%N 2
%P 173-178
%K IVM, IVF, IVC.
%R https://doi.org/10.1071/R97080
%U https://www.publish.csiro.au/paper/R97080 
%X In Experiment 1, to determine the developmental potential of buffalo oocytes of different qualities, compact cumulus&#8211;oocyte complexes (COCs) with an unexpanded cumulus mass, and with homogeneous ooplasm were classified as Grade 1 (with 5 layers of cumulus cells) and Grade 2 less than 4 layers of cumulus cells). Grade-3 oocytes were either without cumulus cells or with expanded cumulus mass, and with irregular ooplasm. The oocytes were matured for 24 h at 38&#183;5&#176;C, 5% CO2 in air in maturation medium (10% fetal bovine serum (FBS) in TCM-199 supplemented with 5 &#181;g mL-1 follicle stimulating hormone-P). The nuclear maturation and cleavage rates, and the proportion of cleaved embryos which developed to morula and blastocyst stage were in the order Grade 1&#62;Grade 2&#62;Grade 3 (P &#60; 0&#183;05). For Experiment 2, the maturation medium consisted of TCM-199 supplemented with one of the following sera at 10% concentration: (1) buffalo oestrus serum (BOS), (2) superovulated buffalo serum (SBS), (3) fetal bovine serum (FBS) and (4) steer serum (SS). After in vitro fertilization (IVF), the oocytes were co-cultured with buffalo oviductal epithelial cells in TCM-199 containing the respective sera at 10% concentration for the subsequent 9 days. The extent of cumulus expansion and nuclear maturation were not different among different groups. The cleavage rates were lower (P &#60; 0&#183;05) with FBS than with BOS, SBS and SS. The proportion of cleaved embryos which developed to blastocyst stage was higher (P &#60; 0&#183;05) with SBS than with BOS, FBS and SS. 
