
TY  - JOUR
A1  - Morris, D. G. 
A1  - Diskin, M. G. 
A1  - Sreenan, J. M. 
T1  - Protein synthesis and phosphorylation by elongating 13&#8211;15-day-old cattle blastocysts
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 2000
VL  - 12
IS  - 2
SP  - 39
EP  - 44
KW  - 
UR  - https://doi.org/10.1071/RD00008 
N2  - Most early embryonic loss in cattle occurs during the period of blastocyst formation and elongation. In this study de novo protein synthesis and phosphorylation by 13&#8211;15-day-old cattle blastocysts was determined. Blastocysts were classified by dimension into spherical, ovoid and elongated stages and cultured under oil for 4 h in drops of KSOM medium containing either 35S-methionine or 32P-orthophosphate. Protein content increased with shape from spherical to ovoid to elongated. There were differences (P&#60;0.05) within and among days in the percentage of radiolabel incorporated. Incorporation of 35S-methionine increased with embryo protein content. However, incorporation of 35S-methionine into TCA-insoluble material, expressed per g of protein, decreased with shape (P&#60;0.001) with more than a 10-fold difference between spherical and elongated 13-day-old blastocysts. Incorporation of 32P-orthophosphate also tended to decrease with shape, with a 3-fold difference between spherical and elongated Day 13 blastocysts. It was concluded that, from Day 13, protein synthesis and phosphorylation, when expressed per unit of protein, decreased from a much higher level, which in the present study was around the time the blastocysts began to elongate. 
ER  -

