
TY  - JOUR
A1  - Dutta, B. 
A1  - Ain, R. 
A1  - Seshagiri, P. B. 
A1  - Karande, A. A. 
T1  - Differential influence of recombinant non-glycosylated and glycosylated glycodelin on human sperm function: comparative studies with hamster spermatozoa
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 2001
VL  - 13
IS  - 3
SP  - 111
EP  - 118
KW  - acrosome reaction; capacitation; egg penetration; sperm.
UR  - https://doi.org/10.1071/RD00119 
N2  - Glycodelin, also known as placental protein 14, has been implicated in endometriosis-related infertility. To determine the role of glycodelin and its glycosylated state, the influence of recombinant nonglycosylated-glycodelin (nongly-glycodelin) and glycosylated-glycodelin (gly-glycodelin) on human sperm function was evaluated. Whereas there was a significant (P&#60;0.001) increase in the capacitation of nongly-glycodelin-treated spermatozoa compared with untreated controls (28.8 1.0% v. 21 1.5% respectively), treatment of spermatozoa with gly-glycodelin markedly (P&#60;0.001) inhibited capacitation (10.7 0. 3%); acrosome reaction (AR) remained unaltered in all treatments. In a zona-free hamster egg penetration assay, the egg penetration index was higher (P&#60;0.001) with nongly-glycodelin-treated spermatozoa (3.4 0.3) than with gly-glycodelin-treated spermatozoa (0.4 0.1) and untreated spermatozoa (1.6 0.2). A similar influence of glycodelin on capacitation was observed with hamster spermatozoa. However, the AR rate was higher (P&#60;0. 01) in nongly-glycodelin-treated spermatozoa (39.4  1.6%) than in either gly-glycodelin-treated spermatozoa (19.3 2.0%) or untreated controls (30.0 1.2%). Moreover, the in vitro fertilization rate was significantly (P&#60;0.01) higher with nongly-glycodelin treated-spermatozoa compared with untreated spermatozoa (77.5 2.3% v. 52.9 4.3%) and gly-glycodelin-treated spermatozoa (38.3 6.5%; P&#60;0. 05). These results indicate that whereas nongly-glycodelin improves, gly-glycodelin inhibits, capacitation and fertilization potential of human and hamster spermatozoa, and that the glycosylation status of glycodelin determines its influence on sperm function. 
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